Journal: Antimicrobial Agents and Chemotherapy

Article Title: In Vitro Activity of LYS228, a Novel Monobactam Antibiotic, against Multidrug-Resistant Enterobacteriaceae

doi: 10.1128/AAC.00552-18

Figure Lengend Snippet: In vitro activity of LYS228 against Enterobacteriaceae isolates

Article Snippet: Bactericidal activity of LYS228 against 271 Enterobacteriaceae isolates table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Organism (β-lactamase) and test agent MIC (μg/ml) Concentration tested (μg/ml) Δlog 10 CFU/ml at: 2 h 4 h 8 h 24 h E. coli ATCC 25922 LYS228 0.25 0.5 −2.0 −4.6 −4.1 2.0 1 −2.2 −4.6 −4.6 −4.6 2 −2.1 −4.6 −4.6 −4.6 Aztreonam 0.12 0.25 −2.1 −3.8 −3.8 −4.4 1 −1.7 −4.1 −4.4 −4.4 Meropenem 0.03 0.06 −1.6 −3.8 −4.4 −4.4 0.25 −3.6 −4.1 −4.4 −4.4 E. coli ATCC 35218 (TEM-1) LYS228 0.06 0.12 −2.8 −3.8 −4.6 −4.9 0.25 −2.8 −4.1 −4.9 −4.9 0.5 −2.9 −3.9 −4.1 −4.9 Meropenem 0.03 0.06 −2.2 −2.9 −4.1 −4.1 0.25 −2.5 −3.0 −4.1 −4.1 E. coli NB27235 (CTX-M-15) LYS228 0.25 0.5 −3.0 −3.1 −4.1 −4.8 1 −3.1 −3.2 −4.2 −4.5 2 −3.5 −3.4 −4.2 −4.8 Meropenem 0.03 0.06 −3.0 −3.6 −1.7 3.7 0.25 −3.3 −4.1 −4.8 −4.8 E. coli NB27169 (KPC-3) LYS228 0.5 1 −0.2 −2.1 −2.6 −4.2 2 −1.0 −2.2 −2.9 −4.9 4 −1.2 −2.0 −2.9 −4.3 E. coli NB27236 (NDM-1) LYS228 4 8 −4.4 −4.4 −4.4 −2.1 16 −2.9 −4.1 −4.4 −4.4 32 −4.4 −3.9 −4.4 −4.4 K. pneumoniae ATCC 43816 LYS228 0.06 0.12 −0.7 −1.9 −0.9 2.7 0.25 −1.0 −1.7 −3.2 −4.6 0.5 −1.1 −2.1 −3.3 −4.6 Aztreonam 0.06 0.12 −0.6 −2.0 −0.5 0.7 0.5 −0.3 −2.1 −3.3 −4.6 Meropenem 0.06 0.12 −2.3 −4.9 −4.9 −4.9 0.5 −3.0 −4.9 −4.9 −4.9 K. pneumoniae NB29254 (SHV-1) LYS228 0.12 0.25 −1.7 −2.6 −4.9 −1.4 0.5 −1.9 −2.9 −4.9 −4.9 1 −1.7 −2.7 −4.9 −4.9 Meropenem 0.03 0.06 −2.3 −3.4 −4.9 −1.8 0.25 −2.8 −3.7 −4.9 −4.9 K. pneumoniae NB29084 (CTX-M-14) LYS228 0.5 1 −0.3 −1.5 −2.7 −4.9 2 −0.9 −2.0 −3.2 −4.9 4 −0.8 −2.4 −3.7 −4.9 Meropenem 0.03 0.06 −1.4 −2.8 −0.7 4.0 0.25 −3.2 −3.5 −1.6 3.9 K. pneumoniae NB29082 (KPC-2) LYS228 0.5 1 −1.4 −2.4 −4.5 −5.0 2 −1.6 −2.5 −4.2 −5.0 4 −1.6 −2.8 −4.1 −5.0 K. pneumoniae ATCC 1100975 (NDM-1) LYS228 1 2 −2.0 −1.9 −3.8 −5.0 4 −1.9 −2.9 −4.1 −5.0 8 −2.0 −1.6 −4.5 −5.0 Open in a separate window Kill kinetics of LYS228 and comparators against E. coli and K. pneumoniae strains expressing various β-lactamases In conclusion, LYS228 is a novel monobactam with potent in vitro activity against a broad panel of clinically relevant Enterobacteriaceae strains, including CRE strains.

Techniques: In Vitro, Activity Assay

Journal: Antimicrobial Agents and Chemotherapy

Article Title: In Vitro Activity of LYS228, a Novel Monobactam Antibiotic, against Multidrug-Resistant Enterobacteriaceae

doi: 10.1128/AAC.00552-18

Figure Lengend Snippet: Bactericidal activity of LYS228 against 271 Enterobacteriaceae isolates

Article Snippet: Bactericidal activity of LYS228 against 271 Enterobacteriaceae isolates table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Organism (β-lactamase) and test agent MIC (μg/ml) Concentration tested (μg/ml) Δlog 10 CFU/ml at: 2 h 4 h 8 h 24 h E. coli ATCC 25922 LYS228 0.25 0.5 −2.0 −4.6 −4.1 2.0 1 −2.2 −4.6 −4.6 −4.6 2 −2.1 −4.6 −4.6 −4.6 Aztreonam 0.12 0.25 −2.1 −3.8 −3.8 −4.4 1 −1.7 −4.1 −4.4 −4.4 Meropenem 0.03 0.06 −1.6 −3.8 −4.4 −4.4 0.25 −3.6 −4.1 −4.4 −4.4 E. coli ATCC 35218 (TEM-1) LYS228 0.06 0.12 −2.8 −3.8 −4.6 −4.9 0.25 −2.8 −4.1 −4.9 −4.9 0.5 −2.9 −3.9 −4.1 −4.9 Meropenem 0.03 0.06 −2.2 −2.9 −4.1 −4.1 0.25 −2.5 −3.0 −4.1 −4.1 E. coli NB27235 (CTX-M-15) LYS228 0.25 0.5 −3.0 −3.1 −4.1 −4.8 1 −3.1 −3.2 −4.2 −4.5 2 −3.5 −3.4 −4.2 −4.8 Meropenem 0.03 0.06 −3.0 −3.6 −1.7 3.7 0.25 −3.3 −4.1 −4.8 −4.8 E. coli NB27169 (KPC-3) LYS228 0.5 1 −0.2 −2.1 −2.6 −4.2 2 −1.0 −2.2 −2.9 −4.9 4 −1.2 −2.0 −2.9 −4.3 E. coli NB27236 (NDM-1) LYS228 4 8 −4.4 −4.4 −4.4 −2.1 16 −2.9 −4.1 −4.4 −4.4 32 −4.4 −3.9 −4.4 −4.4 K. pneumoniae ATCC 43816 LYS228 0.06 0.12 −0.7 −1.9 −0.9 2.7 0.25 −1.0 −1.7 −3.2 −4.6 0.5 −1.1 −2.1 −3.3 −4.6 Aztreonam 0.06 0.12 −0.6 −2.0 −0.5 0.7 0.5 −0.3 −2.1 −3.3 −4.6 Meropenem 0.06 0.12 −2.3 −4.9 −4.9 −4.9 0.5 −3.0 −4.9 −4.9 −4.9 K. pneumoniae NB29254 (SHV-1) LYS228 0.12 0.25 −1.7 −2.6 −4.9 −1.4 0.5 −1.9 −2.9 −4.9 −4.9 1 −1.7 −2.7 −4.9 −4.9 Meropenem 0.03 0.06 −2.3 −3.4 −4.9 −1.8 0.25 −2.8 −3.7 −4.9 −4.9 K. pneumoniae NB29084 (CTX-M-14) LYS228 0.5 1 −0.3 −1.5 −2.7 −4.9 2 −0.9 −2.0 −3.2 −4.9 4 −0.8 −2.4 −3.7 −4.9 Meropenem 0.03 0.06 −1.4 −2.8 −0.7 4.0 0.25 −3.2 −3.5 −1.6 3.9 K. pneumoniae NB29082 (KPC-2) LYS228 0.5 1 −1.4 −2.4 −4.5 −5.0 2 −1.6 −2.5 −4.2 −5.0 4 −1.6 −2.8 −4.1 −5.0 K. pneumoniae ATCC 1100975 (NDM-1) LYS228 1 2 −2.0 −1.9 −3.8 −5.0 4 −1.9 −2.9 −4.1 −5.0 8 −2.0 −1.6 −4.5 −5.0 Open in a separate window Kill kinetics of LYS228 and comparators against E. coli and K. pneumoniae strains expressing various β-lactamases In conclusion, LYS228 is a novel monobactam with potent in vitro activity against a broad panel of clinically relevant Enterobacteriaceae strains, including CRE strains.

Techniques: Activity Assay

Journal: Antimicrobial Agents and Chemotherapy

Article Title: In Vitro Activity of LYS228, a Novel Monobactam Antibiotic, against Multidrug-Resistant Enterobacteriaceae

doi: 10.1128/AAC.00552-18

Figure Lengend Snippet: Kill kinetics of LYS228 and comparators against E. coli and K. pneumoniae strains expressing various β-lactamases

Article Snippet: Bactericidal activity of LYS228 against 271 Enterobacteriaceae isolates table ft1 table-wrap mode="anchored" t5 TABLE 4 caption a7 Organism (β-lactamase) and test agent MIC (μg/ml) Concentration tested (μg/ml) Δlog 10 CFU/ml at: 2 h 4 h 8 h 24 h E. coli ATCC 25922 LYS228 0.25 0.5 −2.0 −4.6 −4.1 2.0 1 −2.2 −4.6 −4.6 −4.6 2 −2.1 −4.6 −4.6 −4.6 Aztreonam 0.12 0.25 −2.1 −3.8 −3.8 −4.4 1 −1.7 −4.1 −4.4 −4.4 Meropenem 0.03 0.06 −1.6 −3.8 −4.4 −4.4 0.25 −3.6 −4.1 −4.4 −4.4 E. coli ATCC 35218 (TEM-1) LYS228 0.06 0.12 −2.8 −3.8 −4.6 −4.9 0.25 −2.8 −4.1 −4.9 −4.9 0.5 −2.9 −3.9 −4.1 −4.9 Meropenem 0.03 0.06 −2.2 −2.9 −4.1 −4.1 0.25 −2.5 −3.0 −4.1 −4.1 E. coli NB27235 (CTX-M-15) LYS228 0.25 0.5 −3.0 −3.1 −4.1 −4.8 1 −3.1 −3.2 −4.2 −4.5 2 −3.5 −3.4 −4.2 −4.8 Meropenem 0.03 0.06 −3.0 −3.6 −1.7 3.7 0.25 −3.3 −4.1 −4.8 −4.8 E. coli NB27169 (KPC-3) LYS228 0.5 1 −0.2 −2.1 −2.6 −4.2 2 −1.0 −2.2 −2.9 −4.9 4 −1.2 −2.0 −2.9 −4.3 E. coli NB27236 (NDM-1) LYS228 4 8 −4.4 −4.4 −4.4 −2.1 16 −2.9 −4.1 −4.4 −4.4 32 −4.4 −3.9 −4.4 −4.4 K. pneumoniae ATCC 43816 LYS228 0.06 0.12 −0.7 −1.9 −0.9 2.7 0.25 −1.0 −1.7 −3.2 −4.6 0.5 −1.1 −2.1 −3.3 −4.6 Aztreonam 0.06 0.12 −0.6 −2.0 −0.5 0.7 0.5 −0.3 −2.1 −3.3 −4.6 Meropenem 0.06 0.12 −2.3 −4.9 −4.9 −4.9 0.5 −3.0 −4.9 −4.9 −4.9 K. pneumoniae NB29254 (SHV-1) LYS228 0.12 0.25 −1.7 −2.6 −4.9 −1.4 0.5 −1.9 −2.9 −4.9 −4.9 1 −1.7 −2.7 −4.9 −4.9 Meropenem 0.03 0.06 −2.3 −3.4 −4.9 −1.8 0.25 −2.8 −3.7 −4.9 −4.9 K. pneumoniae NB29084 (CTX-M-14) LYS228 0.5 1 −0.3 −1.5 −2.7 −4.9 2 −0.9 −2.0 −3.2 −4.9 4 −0.8 −2.4 −3.7 −4.9 Meropenem 0.03 0.06 −1.4 −2.8 −0.7 4.0 0.25 −3.2 −3.5 −1.6 3.9 K. pneumoniae NB29082 (KPC-2) LYS228 0.5 1 −1.4 −2.4 −4.5 −5.0 2 −1.6 −2.5 −4.2 −5.0 4 −1.6 −2.8 −4.1 −5.0 K. pneumoniae ATCC 1100975 (NDM-1) LYS228 1 2 −2.0 −1.9 −3.8 −5.0 4 −1.9 −2.9 −4.1 −5.0 8 −2.0 −1.6 −4.5 −5.0 Open in a separate window Kill kinetics of LYS228 and comparators against E. coli and K. pneumoniae strains expressing various β-lactamases In conclusion, LYS228 is a novel monobactam with potent in vitro activity against a broad panel of clinically relevant Enterobacteriaceae strains, including CRE strains.

Techniques: Expressing, Concentration Assay